Epigallocatechin-3-gallate interferes with EBV-encoding AP-1 signal transduction pathway / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 393-397, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-254326
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the interference effect of Epigallocatechin-3-Gallate (EGCG) on targets of Activator Protein-1 (AP-1) signal transduction pathway activated by EB virus encoded latent membrane protein 1 in nasopharyngeal carcinoma (NPC) cell lines.</p><p><b>METHODS</b>Survival rate of cells was determined by MTT assay. AP-1 and CyclinD1 activation were analyzed by promoter luciferase reporter system. Nuclear translocation of JNK was analyzed by indirect immunofluorescence. Protein expression and phosphorylation were observed by Western blot.</p><p><b>RESULTS</b>EGCG inhibited the survival of CNE1 and CNE-LMP1 cells and the activity of AP-1 caused by LMP1 in CNE-LMP1 cells. EGCG also inhibited the nuclear translocation of JNK and the phosphorylation of c-Jun. It also inhibited cyclinD1 promoter activity and cyclinD1 expression.</p><p><b>CONCLUSION</b>EGCG inhibits AP-1, JNK, c-Jun and cyclinD1 which are key targets on AP-1 signal transduction pathway. The results may explain the molecular mechanism of action of EGCG against nasopharyngeal carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Phosphorylation
/
Physiology
/
Virology
/
Carcinoma, Squamous Cell
/
Signal Transduction
/
Catechin
/
Cell Survival
/
Viral Matrix Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2004
Type:
Article
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