Nimesulide, a selective cyclooxygenase-2 inhibitor inhibits telomerase activity by blocking activation of PKB in gastric cancer cell line / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 209-212, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-254340
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of nimesulide, a selective COX-2 inhibitor, on cell viability, telomerase and PKB activities in human gastric cancer cell line SGC7901 and to explore its molecular mechanism of selective growth inhibition.</p><p><b>METHODS</b>MTT assay was used to determine cell viability after incubation for 0, 12, 24, and 48 h in different concentrations (0, 25, 50, 100, 200 micro mol/L) of nimesulide and/or okadaic acid (300 nmol/L). Telomerase and protein kinase B (PKB) activities were detected using TRAP PCR-ELISA and nonradioactive IP-kinase assay.</p><p><b>RESULTS</b>Nimsulide caused a time and dose-dependent reduction of cell numbers of SGC7901. The telomerase and PKB activities were significantly inhibited, and the inhibition of telomerase activity was partly associated with decrease in PKB activity.</p><p><b>CONCLUSION</b>Selective COX-2 inhibitor nimesulide inhibits telomerase activity of gastric cancer cells by partly blocking the activation of protein kinase B. The results suggest an additional signaling pathway underlying the anti-cancer effect of COX-2 inhibitor.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Stomach Neoplasms
/
Sulfonamides
/
Time Factors
/
Adenocarcinoma
/
Proto-Oncogene Proteins
/
Cyclooxygenase Inhibitors
/
Protein Serine-Threonine Kinases
/
Telomerase
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2004
Type:
Article
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