Expression and significance of Beclin-1 in vasculogenic mimicry formation of gastric cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 716-719, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-254430
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect and mechanism of autophagy specific gene Beclin-1 in gastric cancer cell SGC7901 on vasculogenic mimicry (VM) forming ability.</p><p><b>METHODS</b>Plasmid vectors with and without integrated shRNA were transfected respectively into SGC7901 cell line (Beclin1-inhibited group and negative control group). Simple SGC7901 cell line was used as blank group. RT-PCR and Western blot were performed to examine the expression of Beclin-1 in 3 groups. Culture was used to construct the VM model in vitro. Different VM forming ability was measured and genes (beclin-1, notch-1) expression of each group was detected before and after VM formation.</p><p><b>RESULTS</b>Beclin-1 and notch-1 expression increased significantly in the process of VM forming. When beclin-1 was inhibited, the formation of VM was limited and VM formative genes expression decreased. As compared to cells of negative control group, those of Beclin1-inhibited group had less number of VM forming cellular tube-like construction (15.4±1.1 vs. 37.8±1.9, P<0.05), shorter length of such construction [(316.8±24.6) mm vs. (385.1±14.2) mm, P<0.05], and less crossing point (11.6±1.1 vs. 27.2±1.1, P<0.05).</p><p><b>CONCLUSIONS</b>Beclin-1 can promote VM formation through maintaining stable expression of gastric cancer cell VM regulating genes. Beclin-1 inhibition may be a new target for gastric cancer gene therapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autophagy
/
Stomach Neoplasms
/
Transfection
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Apoptosis Regulatory Proteins
/
Beclin-1
/
Genetic Vectors
/
Membrane Proteins
/
Metabolism
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2014
Type:
Article
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