Association of CFTR gene polymorphism with congenital bilateral absence of vas deferens in ethnic Han Chinese patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 729-732, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-254526
ABSTRACT
<p><b>OBJECTIVE</b>To assess the association between a 5T polymorphism in intron 8 of cystic fibrosis transmembrane conductance regulator (CFTR) gene and congenital bilateral absence of vas deferens (CBAVD) in Han Chinese males.</p><p><b>METHODS</b>Genomic DNA from 33 individuals with CBAVD and 99 azoospermic males with CBAVD were recruited. The 5T polymorphism was detected with PCR, TA cloned and sequenced.</p><p><b>RESULTS</b>CFTR gene mutations were identified in 17 (51.5%) of patients with CBAVD. In 3 patients (17.6%), the mutations were identified on both alleles. Nine CFTR gene mutations (9.1%) were detected in 99 azoospermic patients, for whom none had mutations on both alleles.</p><p><b>CONCLUSION</b>This study has confirmed molecular heterogeneity of CFTR mutations in CBAVD. For CBAVD patients without 5T mutations, other changes may be found in the same gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Congenital Abnormalities
/
Vas Deferens
/
Introns
/
Cystic Fibrosis Transmembrane Conductance Regulator
/
Genetic Predisposition to Disease
/
Asian People
/
Alleles
/
Male Urogenital Diseases
/
Genetics
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2013
Type:
Article
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