DNA extraction and sex determination for human teeth dated 3000 years ago unearthed in Xi'an / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 619-621, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-254549
ABSTRACT
<p><b>OBJECTIVE</b>To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi'an and determine the genders for the individuals.</p><p><b>METHODS</b>Thirty five ancient human teeth were studied. A 'Reverse-root-canal' technique and a Chelex-100 solution were used to extract the DNA. Specific primers for Amelogenin gene were designed for PCR amplification.</p><p><b>RESULTS</b>Genomic DNA was successfully extracted from 30 samples, for which 8 were determined to be males and 22 were females.</p><p><b>CONCLUSION</b>The 'Reverse-root-canal' technique may be used for extracting DNA from ancient human teeth. Genetics method can supplement physical anthropology for determination of sex for ancient samples.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paleodontology
/
Sex Determination Analysis
/
Tooth
/
DNA
/
Chemistry
/
China
/
Polymerase Chain Reaction
/
History, Ancient
/
Amelogenin
/
Genetics
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2013
Type:
Article
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