Effect of agmatine on proliferation of rat PASMCs induced by serum / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To determine the effect of agmatine on the proliferation of PASMCs induced by serum.</p><p><b>METHODS</b>Primary culture of rat PASMCs was prepared from adult male Wistar rat pulmonary artery by the method of tissue block anchorage. PASMCs were divided into two groups control group and agmatine administration group. The activity of LDH in the medium was measured by chromatometry. The 3H-TdR incorporation was measured by liquid scintillometry. The cell cycle was measured by flow cytometry. The PCNA content was measured by image analysis.</p><p><b>RESULTS</b>Agmatine did not exert significant effect on the activity of LDH in the medium. Agmatine significantly decreased the 3H-TdR incorporation and PCNA content of PASMCs. Agmatine significantly decreased the cell ratio of G2/M phase and increased the cell ratio of G0/G1 phase. With the increment of the concentration of agmatine, 3H-TdR incorporation was significantly decreased correspondingly.</p><p><b>CONCLUSION</b>Agmatine has no significant cytotoxic effect on PASMCs. Agmatine can dose-dependently inhibit the proliferation of rat PASMCs induced by serum.</p>