Effects of L-arginine and aminoguanidine on the endotoxin induced lung injury in rats / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To investigate the effects of L-arginine and nitric oxide synthase (NOS) inhibitor Aminoguanidine (AG) on endotoxin induced lung injury in rats.</p><p><b>METHODS</b>Forty eight healthy male SD rats weighing (300 +/- 20) g were used. The animals were anesthetized with 20% urethane 1 g x kg(-1). Common carotid artery (CAA) and common carotid vein (CAV) were exposed through a median incision in the neck. Mean arterial pressure (MAP) was measured through a pressure transducer connected with intubation of CAA. The animals were randomly divided into six groups group 1 control group 2 LPS (5 mg x kg(-1) intravenous injection, i.v.); group 3 AG (50 mg x kg(-1) intraperitoneal injection, IP); group 4 high dose L-arginine (500 mg x kg(-1), IP); group 5 low dose L-arginine (250 mg x kg(-1) IP). Group 6 L-arginine + AG (250 mg x kg(-1), 50 mg x kg(-1), IP). Group 1 The animals were killed 6 h after 0.9% saline solution was given. Group 2 0.9% saline solution was given 3 h after LPS i.v. and the animals were killed 3 h after medication. Group 3, 4, 5 and 6 AG, L-arginine and L-arginine+ AG were given 3 h after LPS i.v. respectively and the animals were killed 3 h after medication respectively. The pulmonary was removed immediately. The pulmonary coefficient and water content in pulmonary tissue were calculated (%). The NO content in plasma, MDA content and NOS, SOD activity in the pulmonary tissue were measured.</p><p><b>RESULTS</b>L-arginine, AG and L-arginine + AG significantly decreased pulmonary coefficient and water content in pulmonary tissue and ameliorated endotoxin induced lung injury. AG and L-arginine + AG significantly decreased NO content in plasma, decreased MDA content and inhibited NOS activity and enhanced SOD activity in the pulmonary tissue.</p><p><b>CONCLUSION</b>It may be concluded that L-arginine, AG and L-arginine + AG have beneficial effects on lung injury induced by LPS.</p>