An experimental study on repair of peripheral nerve injury by transplantation of microcapsulated NGF-expressing NIH 3T3 cells / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 53-57, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-255104
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of promoting nerve regeneration by using microcapsulated NGF-expressing cells transplantation.</p><p><b>METHODS</b>The plasmid pcDNA3. 1 + /NGF, containing rat NGF gene, was transfected into the NIH 3T3 cell by using FuGENE6 transfection reagent. The genetically modified cells expressing NGF gene were enclosed within the alginate-polylysine-alginate (APA) microcapsules and then cultured in vitro. The growth and NGF secretion of the cells were measured periodically. At the same time, these microcapsulated NGF-expressing cells were transplanted into the injured sciatic nerve. The regeneration and functional recovery of the nerve were evaluated in 4 weeks, 8 weeks and 12 weeks after the operation.</p><p><b>RESULTS</b>The microcapsulated cells had survived and secreted the NGF in three months in vitro. In the group with microcapsulated NGF-expressing cells, the number of the regenerated axons was in large and the nerve fibers were arranged regularly. Compared to other groups, there was less scar , edema and monocytes found at the stoma in the goup. The moter nerve conductive velocity, nerve muscle-action potential and SFI were improved.</p><p><b>CONCLUSIONS</b>The microcapsulated NGF-expressing cells could significantly enhance the nerve regeneration and reduce inflammatory response of xenograft.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Rats, Sprague-Dawley
/
Cell Transplantation
/
Nerve Growth Factor
/
NIH 3T3 Cells
/
Genetics
/
Nerve Regeneration
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2005
Type:
Article
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