The effects of aloesin and arbutin on cultured melanocytes in a synergetic method / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 369-371, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-255143
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of aloesin and arbutin on normal cultured human melanocytes in synergetic method.</p><p><b>METHODS</b>Building up the system of cultured human melanocytes. The cultured melanocytes in vitro were treated with the mixture of aloesin and arbutin. The cell viability and tyrosinase activity was measured by MTT assay, utilization of L-Dopa as the substrate respectively; melanin content was measured by image analysis system. Furthermore, the effects of the mixture on melanocytes were compared with that of aloesin and arbutin.</p><p><b>RESULTS</b>The mixture of aloesin and arbutin showed an inhibition on tyrosinase activity of human melanocytes and reduced significantly melanin content. Between the mixture and the single use of aloesin or arbutin, there is significant difference (P < 0.05). On the other hand, the mixture has little influence on melanocytes viability and there is negative significance.</p><p><b>CONCLUSION</b>The mixture of aloesin and arbutin can significantly inhibit the tyrosinase activity and melanogenesis of cultured human melanocytes. It showed the effects of aloesin and arbutin in a synergistic manner. It is worth to give farther study later.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Arbutin
/
Cells, Cultured
/
Chromones
/
Monophenol Monooxygenase
/
Cell Biology
/
Dose-Response Relationship, Drug
/
Drug Synergism
/
Glucosides
/
Melanocytes
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2004
Type:
Article
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