Expression of plateau adaptation gene of rat tissues after plain acute exposure to high altitude / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 571-577, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-255152
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of the plateau adaptablity gene(EPAS1, EGLN1 and PPARα) and proteins(HIF-2, PHD2 and PPARα) in rats blood, heart, liver, lung and kidney tissue after the rats exposed to high altitude.</p><p><b>METHODS</b>The Wistar rats were randomly divided into plain group(Shanghai, 55 m), acute exposure to high altitude 3400 m group, acute exposure to high altitude 4300 m group. Blood and organs of rats were collected in 1, 3, 5 days after arrival. Real time PCR and ELISA were used to compare the expression of plateau adaptablity gene and related protein between plain group and high altitude exposure groups.</p><p><b>RESULTS</b>The count of red blood cells, hemoglobin and HCT in high altitude 4300 m were higher than those in plain group. Compared with plain group, the expression of EPAS1 gene in blood, heart, liver and kidney tissue of rats at high altitude increased obviously(all P<0.05); the expression of EGLN1 in the heart, liver, brain and kidney increased, and PPARα gene in the heart, liver and kidney increased(all P<0.05). Compared with plain group, the expression of HIF-2 protein increased significantly at high altitudes in the liver, brain and kidney tissues. PHD2 and PPARα increased in the heart, liver and kidney.</p><p><b>CONCLUSION</b>Plateau adaptive genes(EPAS1, EGLN1 and PPARα) and protein(HIF-2, PHD2 and PPARα) differed in different altitude and different organizations. They might be used as target markers of plateau hypoxia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Adaptation, Physiological
/
China
/
Rats, Wistar
/
Procollagen-Proline Dioxygenase
/
PPAR alpha
/
Basic Helix-Loop-Helix Transcription Factors
/
Altitude
/
Hypoxia-Inducible Factor-Proline Dioxygenases
/
Heart
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2015
Type:
Article
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