Neuroprotective effects of paeonol in a cell model of Parkinson disease / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 30-36, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-255238
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of paeonol on neuron cell model of Parkinson disease (PD).</p><p><b>METHODS</b>The cell model of Parkinson disease was induced by treatment of 1-Methyl-4-phenylpyridinium (MPP+) in PC12 cells, the PD model cells were treated with 1 μmol/L, 3 μmol/L or 9 μmol/L paeonol for 24h, respectively. Cell viability and LDH leakage were detected by MTT and lactate dehydrogenase (LDH) assay; the apoptosis of PC12 cells was assessed by Hoechst 33258 staining and flow cytometry; reactive oxygen species (ROS) production was detected by DCFH-DA method; and the ratio of Bax/Bcl-2 and activation of caspase-3 were determined by Western blotting.</p><p><b>RESULTS</b>MPP+ treatment significantly reduced cell viability, increased LDH leakage, enhanced the proportion of apoptotic cells and ROS production. In addition, MPP+ treatment dramatically increased the Bax/Bcl-2 ratio, and the activation of caspase-3. Compared to PD model group, paeonol treatment significantly enhanced cell viability, decreased LDH leakage, inhibited the proportion of apoptotic cells and ROS production, reduced the Bax/Bcl-2 ratio and the activated caspase-3 protein.</p><p><b>CONCLUSION</b>Paeonol can prevent PC12 cells from apoptosis induced by MPP+, and the mechanism may be associated with the down-regulation of ROS production, Bax/Bcl-2 ratio and Caspase-3 activation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parkinson Disease
/
Acetophenones
/
Pharmacology
/
Down-Regulation
/
1-Methyl-4-phenylpyridinium
/
Cell Survival
/
PC12 Cells
/
Reactive Oxygen Species
/
Apoptosis
/
Neuroprotective Agents
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2015
Type:
Article
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