Periodic quantity variation of proliferating neuronal progenitors in adult rats after global brain ischemia / 南方医科大学学报
Journal of Southern Medical University
;
(12): 564-569, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-255253
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the periodic quantity variation of proliferating neuronal progenitors after global brain ischemia and provide evidence for choosing the time-window of drug therapy to promote neuronal regeneration after ischemia.</p><p><b>METHODS</b>Adult male Wistar rats were subjected to 15-min global brain ischemia (four-vessel occlusion model) and randomized subsequently into 8 groups (n=3). The rats were given intraperitoneal injections of BrdU (75 mg/kg) for 4 times daily (at a 2-hour interval) since day 7 till day 11 after ischemia, and on day 29, the rats were perfused transcardially for fixation. Another 3 normal rats were given BrdU in the same manner and killed the next day. Coronal sections of the brain tissue (30 microm) were prepared for immunocytochemical detection of BrdU-labeled cells and immunofluorescent detection of BrdU/NeuN double-labeled cells. The density of BrdU-positive cells and BrdU/NeuN double-labeled cells in the hippocampal dentate gyrus (DG) and CA1 region were counted and the density of proliferating cells at different days after ischemia were compared using one-way ANOVA.</p><p><b>RESULTS</b>The proliferation of the neuronal progenitors increased after global brain ischemia. The number of BrdU-positive cells in the DG and CA1 region decreased gradually in 7-10 days after ischemia, and reached the normal level during 11-14 days. The differentiation of the progenitors did not vary after ischemia.</p><p><b>CONCLUSION</b>Increased proliferation of the neuroprogenitors occurs mainly within the initial 10 days after global ischemia in rats.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Stem Cells
/
Time Factors
/
Random Allocation
/
Cell Differentiation
/
Brain Ischemia
/
Rats, Wistar
/
Cell Proliferation
/
Nerve Regeneration
/
Neurons
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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