Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophages / 南方医科大学学报
Journal of Southern Medical University
;
(12): 558-560, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-255255
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of advanced oxidation protein products (AOPP) on nitric oxide (NO) production in mouse peritoneal macrophages (MPMs).</p><p><b>METHODS</b>MPMs were incubated in the absence or presence of lipopolysaccharide (LPS) with AOPP-modified bovine serum albumin (BSA) prepared by exposure of BSA to hypoclorous acid or pre-treated with AOPP-BSA and subsequent stimulation with LPS. NO production in the supernatants of the culture media was determined spectrophotometrically using Griess method. The cell viability was measured by MTT assay.</p><p><b>RESULTS</b>BSA induced significant NO production in MPMs. AOPP modification of BSA significantly inhibited NO production, and AOPP-BSA exhibited time- and dose-dependent inhibition of NO production induced by LPS in MPMs incubated together with LPS or pre-treated before LPS stimulation.</p><p><b>CONCLUSION</b>AOPP-BSA is capable of inhibiting inducible NO production in MPMs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidation-Reduction
/
Pharmacology
/
Time Factors
/
Serum Albumin, Bovine
/
Cell Survival
/
Cells, Cultured
/
Chemistry
/
Lipopolysaccharides
/
Reactive Oxygen Species
/
Glycation End Products, Advanced
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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