Effect of estrogen on osteoprotegerin, osteoclast differentiation factor and macrophage colony stimulating factor mRNA expressions in ovariectomized rat bone tissue / 南方医科大学学报
Journal of Southern Medical University
;
(12): 532-534, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-255261
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of the estrogen on the mRNA expression of osteoprotegerin (OPG), osteoclast differentiation factor (ODF) and macrophage colony stimulating factor (M-CSF) in bone tissue of ovariectomized rats, and investigate the possible pathway of estrogen in preventing and treating postmenopausal osteoporosis. METHODS; Thirty healthy adult SD rats were randomly divided into sham operation group, ovariectomized group and estrogen-treated group. All rats were ovariectomized except those in the sham operation group. Bone density of the L3-L6 vertebra was detected 12 weeks after the operation. The total RNA were extracted from the femur to examine mRNA expression of OPG, ODF and M-CSF by real-time PCR.</p><p><b>RESULTS</b>Estrogen increased the bone density of the ovariectomized rat lumbar vertebra and up-regulated the expression of OPG, whereas down-regulated the expression of M-CSF and lowered ODFOPG ratio.</p><p><b>CONCLUSION</b>The effect of estrogen in treating postmenopausal osteoporosis is closely correlated with the regulation of OPG and M-CSF expressions and ODFOPG ratio.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoporosis
/
Pharmacology
/
Bone and Bones
/
RNA, Messenger
/
Ovariectomy
/
Macrophage Colony-Stimulating Factor
/
Rats, Sprague-Dawley
/
Therapeutic Uses
/
Drug Therapy
/
Estrogens
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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