Sodium transport and its regulation of acutely isolated alveolar type II cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To observe the sodium current of acutely isolated alveolar type II cells and its regulation.</p><p><b>METHODS</b>After isolation the AT II cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect of amiloride and terbutaline on the current was investigated.</p><p><b>RESULTS</b>The amiloride-sensitive current of AT II cells were recorded, which could be obviously stimulated by terbutaline.</p><p><b>CONCLUSION</b>Acutely isolated AT II cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions.</p>