Detection of mdr1 gene by real-time fluorescence quantitative polymerase chain reaction using Taq Man-MGB probe / 南方医科大学学报
Journal of Southern Medical University
;
(12): 466-468, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-255283
ABSTRACT
Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061 x 10(3) to 3.061 x 10(9) cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymerase Chain Reaction
/
DNA Primers
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Genes, MDR
/
Taq Polymerase
/
Fluorescent Dyes
/
Fluorometry
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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