Morphological changes of ECV304 cells infected by herpes simplex virus type 2 / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To observe the pathological changes and morphological alterations of ECV304 cells after the infection by herpes simplex virus type 2 (HSV-2) in vitro.</p><p><b>METHODS</b>Passaged ECV304 cells were infected with HSV-2, TCID50 and morphological changes were observed by optical microscopy and tissue staining.</p><p><b>RESULTS</b>One day after HSV-2 infection, swelling, rounding, and increase of thickened cytoplasmic granules occurred in the ECV304 cells, and on day 2, cell fusion was observed with weakened nuclear staining.</p><p><b>CONCLUSION</b>ECV304 cells mostly undergo necrosis after HSV-2 infection without obvious evidence of cell apoptosis.</p>