Expanded T cell clones and cytokines expression in human gastric carcinomas of different histological types / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 120-123, 2002.
Article
in English
| WPRIM
| ID: wpr-255416
ABSTRACT
<p><b>OBJECTIVE</b>To study the characteristics of host immune response against human gastric carcinoma of different histological types.</p><p><b>METHODS</b>The expression of 24 families of T cell receptor beta chain variable region (TCRVbeta) and cytokine profiles in isolated CD4(+) and CD8(+) subsets, as well as the cytokine profiles in purified epithelial cells from the tumor tissue and the residual benign tissue of patients with gastric carcinoma, was detected by a highly sensitive radioactivity labeled semi-quantitative RT-PCR technique.</p><p><b>RESULTS</b>The number of expanded T cell clones in CD8(+) subset from tumor tissue of the intestinal-type carcinoma was larger than that of diffuse-type (P = 0.046). The mRNA levels of IL-6, IL-8 in CD8(+) T subset, as well as the level of TNF-alpha in CD4(+) T subset from the tumor tissue of the diffuse type (0.61 +/- 0.29, 0.56 +/- 0.22, 0.09 +/- 0.03) were significantly higher than that from the residual benign tissue (0.14 +/- 0.05, 0.27 +/- 0.09, 0.04 +/- 0.02; P = 0.028, P = 0.043, P = 0.046). However, the mRNA level of IL-8 in CD8(+) subset and epithelial tumor cells of the intestinal-type (0.57 +/- 0.25, 0.27 +/- 0.07) was significantly higher than that from the residual benign tissue (0.21 +/- 0.07, 0.14 +/- 0.06; P = 0.028, P = 0.028).</p><p><b>CONCLUSIONS</b>The characteristics of host immune response against tumor are different between intestinal-type and diffuse-type gastric carcinoma. Both fewer expanded T cell clones and more suppressive cytokines suggest a more suppressive immune status in the local tumor lesion of diffuse-type than in the intestinal-type of the gastric carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Stomach Neoplasms
/
RNA, Messenger
/
T-Lymphocytes
/
CD4-Positive T-Lymphocytes
/
Gene Expression Regulation, Neoplastic
/
Cytokines
/
Interleukin-8
/
Transforming Growth Factor beta
/
Interleukins
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Chinese Journal of Pathology
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS