Your browser doesn't support javascript.
loading
Association between peroxisome proliferator-activated receptor-gamma coactivator-1alpha gene polymorphisms and type 2 diabetes in southern Chinese population: role of altered interaction with myocyte enhancer factor 2C / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1878-1885, 2007.
Article in English | WPRIM | ID: wpr-255484
ABSTRACT
<p><b>BACKGROUND</b>Some single nucleotide polymorphisms (SNPs) in the peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha gene have been reported to be associated with type 2 diabetes in different populations, and studies on Chinese patients yielded controversial results. The objective of this case-control study was to explore the relationship between SNPs of PGC-1alpha and type 2 diabetes in the southern Chinese population and to determine whether the common variants Gly482Ser and Thr394Thr, in the PGC-1alpha gene have any impacts on interaction with myocyte enhancer factor (MEF) 2C.</p><p><b>METHODS</b>The SNPs in all exons of the PGC-1alpha gene was investigated in 50 type 2 diabetic patients using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and direct sequencing. Thereafter, 263 type 2 diabetic patients and 282 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A bacterial two-hybrid system and site-directed mutagenesis were used to investigate whether Gly482Ser and Thr394Thr variants in the PGC-1alpha gene alter the interaction with MEF2C.</p><p><b>RESULTS</b>Three frequent SNPs (Thr394Thr, Gly482Ser and Thr528Thr) were found in exons of the PGC-1alpha gene. Only the Gly482Ser variant had a different distribution between diabetic patients and healthy subjects, with the 482Ser allele more frequent in patients than in controls (40.1% vs 29.3%, P < 0.01). Even in controls, the 482Ser (A) carriers were more likely to have higher levels of total cholesterol and low-density lipoprotein cholesterol than the 482Gly (G) carriers. The 394A-482G-528A haplotype was associated with protection from diabetes, while the 394A-482A-528A was associated with the susceptibility to diabetes. The bacterial two-hybrid system and site-directed mutagenesis revealed that the 482Ser variant was less efficient than the 482Gly variant to interact with MEF2C, whereas the 394Thr (A) had a synergic effect on the interaction between 482Ser variant and MEF2C.</p><p><b>CONCLUSIONS</b>The results suggested that the 482Ser variant of PGC-1alpha conferred the susceptibility to type 2 diabetes in the southern Chinese population. The underlying mechanism may be attributable, at least in part, to the altered interaction between the different variants (Gly482Ser, Thr394Thr) in the PGC-1alpha gene and MEF2C.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Protein Binding / Transcription Factors / China / Polymerase Chain Reaction / Myogenic Regulatory Factors / Polymorphism, Single-Stranded Conformational / Polymorphism, Single Nucleotide / Asian People / Diabetes Mellitus, Type 2 / Ethnology Type of study: Observational study / Risk factors Limits: Aged / Female / Humans / Male Country/Region as subject: Asia Language: English Journal: Chinese Medical Journal Year: 2007 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Protein Binding / Transcription Factors / China / Polymerase Chain Reaction / Myogenic Regulatory Factors / Polymorphism, Single-Stranded Conformational / Polymorphism, Single Nucleotide / Asian People / Diabetes Mellitus, Type 2 / Ethnology Type of study: Observational study / Risk factors Limits: Aged / Female / Humans / Male Country/Region as subject: Asia Language: English Journal: Chinese Medical Journal Year: 2007 Type: Article