Beneficial effects of hydrocortisone in induced acute pancreatitis of rats / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1757-1761, 2007.
Article
in English
| WPRIM
| ID: wpr-255510
ABSTRACT
<p><b>BACKGROUND</b>Little is known of the effects of hydrocortisone on cell adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and its counterreceptors (LFA-1, Mac-1) in acute pancreatitis (AP). We investigated the effects of prior treatment with hydrocortisone on the production of ICAM-1 and its counterreceptors (LFA-1 and Mac-1) in AP of rats to clarify the effect of hydrocortisone on induced acute pancreatitis.</p><p><b>METHODS</b>Acute pancreatitis was induced by infusion of 5% chenodeoxycholic acid into the pancreatic duct, followed by ligation of pancreatic duct. Before induction of acute pancreatitis, rats were treated with hydrocortisone (n = 20) or 0.9% saline (n = 20). Blood and specimens from pancreas and lung were obtained from 5 rats from each treatment euthanized at 1 hour or 3 hours, 6 hours, 12 hours. Expression of ICAM-1 was assessed by immunohistochemistry and Western blot analysis of pancreas and lungs. The expression of LFA-1 and Mac-1 on neutrophils was detected by flow cytometer. The therapeutic effect of hydrocortisone was assessed from injuries to pancreas and lung.</p><p><b>RESULTS</b>ICAM-1 expression in the pancreas of hydrocortisone group was significantly less than in control group at 3 hours and 6 hours. In the lungs of hydrocortisone group, ICAM-1 expression was significantly less than in control group at 3 hours, 6 hours and 12 hours. The expression of LFA-1 and Mac-1 on neutrophils in blood increased significantly in control group over hydrocortisone group. Increased expression of ICAM-1, LFA-1 and Mac-1 preceded leukocyte infiltration. Compared to untreated animals with acute pancreatitis, rats pretreated with hydrocortisone had significantly reduced histological lung injury and output of ascitic fluid.</p><p><b>CONCLUSIONS</b>Prior treatment with hydrocortisone before the induction of acute pancreatitis ameliorates pulmonary injury and the output of ascitic fluid and reduces the expression of ICAM-1 and its counterreceptors (LFA-1, Mac-1) in acute pancreatitis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatitis
/
Pharmacology
/
Blood
/
Hydrocortisone
/
Acute Disease
/
Lymphocyte Function-Associated Antigen-1
/
Macrophage-1 Antigen
/
Rats, Sprague-Dawley
/
Intercellular Adhesion Molecule-1
/
Therapeutic Uses
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2007
Type:
Article
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