Growth of breast cancer cells is inhibited in vitro by cyclin E-siRNA / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 170-173, 2009.
Article
in Zh
| WPRIM
| ID: wpr-255537
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes of biological characteristics of breast cancer cell line by cyclin E expression.</p><p><b>METHODS</b>Human breast cancer cell line MCF-7 was transfected with cyclin E siRNA vector pEGFP/CCNE2. siRNA-induced silencing of cyclin E was determined by RT-PCR at RNA level and Western blot at protein level. The proliferation of MCF-7 cells and their sensitivity to chemotherapy was measured by CCK-8 assay. The cells were examined by FCM. The cell line was injected into nude mice and the tumor size was measured.</p><p><b>RESULTS</b>The expression of cyclin E was inhibited in the MCF-7 cells. The relative expression level of cyclin E mRNA was 0.23 +/- 0.05, and that of cyclin E protein was 0.24 +/- 0.05. The cell growth was inhibited by 68.56% +/- 0.08%, and their sensitivity to chemotherapy was increased. Most cells were blocked at G(1) (77.38%), their tumorigenic ability in nude mice was reduced, and the size of tumor formed in mice of the experimental group was decreased than that of controls.</p><p><b>CONCLUSION</b>Inhibition of cyclin E expression in breast cancer cells can block their cell cycle at G(1) phase, reduce their cell growth, differentiation and proliferation, and increase their sensitivity to chemotherapy.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Plasmids
/
Breast Neoplasms
/
RNA, Messenger
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Transfection
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Doxorubicin
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Cell Cycle
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Paclitaxel
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Cyclin E
Limits:
Animals
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Female
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Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2009
Type:
Article