Tree analysis pattern of mass spectral urine profiles in differential diagnosis of bladder transitional cell carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 274-277, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-255665
ABSTRACT
<p><b>OBJECTIVE</b>To develope a tree analysis pattern of mass spectral urine profiles to discriminate bladder transitional cell carcinoma (TCC) from non-cancer lesions using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology.</p><p><b>METHODS</b>Urine samples from 61 bladder transitional cell carcinoma (TCCs) patients, 53 healthy volunteers and 42 patients with other urogenital diseases were analyzed using IMAC-Cu-3 ProteinChip. Proteomic spectra were generated by SELDI-TOF- MS. A preliminary "training" set of spectra derived from analysis of urine from 46 TCC patients, 32 patients with benign urogenital diseases (BUD), and 40 age-matched unaffected healthy men were used to train and develop a decision tree classification algorithm which identified a fine-protein mass pattern that discriminated cancers from non-cancers effectively. A blinded test set including 38 cases was used to determine the sensitivity and specificity of the classification system.</p><p><b>RESULTS</b>The algorithm identified a cluster pattern that, in the training set, segregated cancer from non-cancer with a sensitivity of 84.8% and specificity of 91.7%. The discriminatory pattern was correctly identified. A sensitivity of 93.3% and a specificity of 87% for the blinded test were obtained when compared the TCC versus non-cancers.</p><p><b>CONCLUSION</b>SELDI-TOF-MS technology is a rapid, convenient and high-throughput analyzing method. The urine tree analysis proteomic pattern as a screening tool is effective for differential diagnosis of bladder cancer. More detailed studies are needed to further evaluate the clinical value of this pattern.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostatic Hyperplasia
/
Urine
/
Urinary Bladder Neoplasms
/
Decision Trees
/
Carcinoma, Transitional Cell
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Cystitis
/
Protein Array Analysis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adult
/
Aged
/
Aged80
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article
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