The effects of different transcripts of AML1 on the transactivation of M-CSF-R gene / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 641-644, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-255830
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of AML1A and AML1B, two splicing isoforms of AML1, on the transactivation of macrophage colony-stimulating factor receptor (M-CSF-R), and explore the mechanism of hematopoietic stem cell committed differentiation and leukemogenesis.</p><p><b>METHODS</b>The expressive plasmids of AML1A and AML1B were constructed, and co-transfected into CV-1 cells with a luciferase reporter plasmid containing M-CSF-R promoter. The transactivity of M-CSF-R promoter was assayed by luminometer.</p><p><b>RESULTS</b>AML1B exhibited a distinct transactivity to M-CSF-R promoter with a sequence-specificity and dosage-dependent manner. AML1A showed no any transactivity but antagonized the effect of AML1B, causing marked reduction of M-CSF-R expression.</p><p><b>CONCLUSION</b>An intact structure of AML1 is necessary for transactivation of M-CSF-R. AML1A may interfere with the transactivation of AML1B, and play a key role in the fine regulation of committed differentiation of hematopoietic cell.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Hematopoietic Stem Cells
/
Transfection
/
Gene Expression Regulation, Leukemic
/
Cell Differentiation
/
Transcriptional Activation
/
Cells, Cultured
/
Haplorhini
/
Receptor, Macrophage Colony-Stimulating Factor
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2005
Type:
Article
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