Molecular mechanism of myeloma cell line differentiation induced by 2-methoxyestradiol / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 598-601, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-255835
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular mechanism of differentiation induction by 2-methoxyestradiol (2ME2) of myeloma cell lines.</p><p><b>METHODS</b>Differentiation induction effect on myeloma cell lines LP-1, CZ-1 and NCI-H929 which were incubated with 2ME2 and XBP-1, Blimp-1, pax-5 phosphorothioate antisense oligodeoxynucleotide (ASODN) was evaluated by cell morphology, CD49e expression, quantitation of light chain secretion, and the level of pax-5 and XBP-1 mRNA expression.</p><p><b>RESULTS</b>2ME2 caused morphological, immunophenotypic and the supernatant light chain secretion changes typical of differentiation in all the three myeloma cell lines. 2ME2 up-regulated the XBP-1 mRNA expression. XBP-1 and Blimp-1 ASODNs partially inhibited the differentiation of LP-1, CZ-1, NCI-H929 cells induced by 2ME2; whereas pax-5 ASODN did the contrary. After incubated with pax-5 ASODN for 72 hours, LP-1, CZ-1, NCI-H929 cells exhibited characteristic morphologic feature of differentiation. The expression of CD49e was increased statistically (P < 0.05). Light chain secretion in the supernatant was also increased statistically (P < 0.05). After incubation with Blimp-1 ASODN, the level of XBP-1 mRNA was declined, while the level of pax-5 mRNA increased.</p><p><b>CONCLUSION</b>2ME2 could induce cell differentiation and up-regulate XBP-1 mRNA expression in myeloma cell lines. Blimp-1 could help 2ME2 with inducing differentiation of myeloma cells through downregulating pax-5 mRNA and upregulating XBP-1 mRNA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Transcription Factors
/
RNA, Messenger
/
Cell Differentiation
/
Cell Line, Tumor
/
DNA-Binding Proteins
/
Estradiol
/
X-Box Binding Protein 1
/
Regulatory Factor X Transcription Factors
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2005
Type:
Article
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