MEKK2 regulates the production of interleukin 2 / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 296-298, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-255887
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of MEKK2 on the production of IL-2 in Jurkat cells stimulated by PHA/anti-CD28 antibody.</p><p><b>METHODS</b>The MEKK2 and JNK kinase activities were measured in both dominant negative MEKK2 Jurkat (dnMEKK2 Jurkat) cells and parental Jurkat cells. The AP(1) and IL-2 promotor activities were measured by luciferase activity assay. The IL-2 mRNA and protein were detected by RT-PCR and Western blot.</p><p><b>RESULTS</b>After stimulation by PHA/anti-CD28, JNK was activated in parental Jurkat cells but not in dnMEKK2 Jurkat cells. The luciferase report gene activities of AP1 and IL-2 promotors were increased by 4- and 5-folds in parental cells whereas only by 1 fold in dnMEKK2 Jurkat cells. The level of IL-2 mRNA and IL-2 protein were increased in parental Jurkat cells but not in dnMEKK2 Jurkat cells.</p><p><b>CONCLUSION</b>MEKK2 plays an important role on the production of IL-2 in Jurkat cell stimulated with PHA/anti-CD28 antibody. It is a potential drug target for the treatment of GVHD and autoimmune disease.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Interleukin-2
/
Jurkat Cells
/
MAP Kinase Kinase Kinase 2
/
MAP Kinase Kinase 4
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2005
Type:
Article
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