High intracellular Mg²⁺ affects the activities of L-type calcium channel in guinea- pig ventricular myocytes / 生理学报
Acta Physiologica Sinica
; (6): 718-722, 2014.
Article
in Zh
| WPRIM
| ID: wpr-255982
Responsible library:
WPRO
ABSTRACT
This study is aimed to investigate the effects of high intracellular Mg²⁺ on L-type calcium channel in guinea-pig ventricular myocytes. The cardiomyocytes were acutely isolated with enzyme digestion method. By adopting inside-out configuration of patch clamp technique, single channel currents of the L-type calcium channel were recorded under different intracellular Mg²⁺ concentrations ([Mg²⁺]i). In control group, which was treated with 0.9 mmol/L Mg²⁺, the relative activity of calcium channel was (176.5 ± 34.1)% (n = 7). When [Mg²⁺]i was increased from 0.9 to 8.1 mmol/L (high Mg²⁺ group), the relative activities of calcium channel decreased to (64.8 ± 18.1)% (n = 6, P < 0.05). Moreover, under 8.1 mmol/L Mg²⁺, the mean open time of calcium channel was shortened to about 25% of that under control condition (P < 0.05), but the mean close time of calcium channel was not altered. These results suggest that high intracellular Mg²⁺ may inhibit the activities of L-type calcium channel, which is mainly due to the shortening of the mean open time of single L-type calcium channel.
Full text:
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Index:
WPRIM
Main subject:
Physiology
/
Patch-Clamp Techniques
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Calcium Channels, L-Type
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Myocytes, Cardiac
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Guinea Pigs
/
Magnesium
Limits:
Animals
Language:
Zh
Journal:
Acta Physiologica Sinica
Year:
2014
Type:
Article