The role of Toll-like receptor 4 on inflammation and Aβ formation in cortex astrocytes / 生理学报
Acta Physiologica Sinica
;
(6): 631-638, 2014.
Article
in English
| WPRIM
| ID: wpr-255992
ABSTRACT
To investigate the role and possible molecular mechanism of astrocytes in inflammation and amyloid β-protein (Aβ) formation, in this research, by using LPS to stimulate cultured rat astrocytes in vitro with or without anti-Toll-like receptor 4 (TLR4) antibody pretreatment, we first detected the TLR4, TNF-α, IL-1β, β-amyloid precursor protein (β-APP) and β-site APP clearing enzyme 1 (BACE1) mRNA with real-time PCR, and TLR4, NF-κB/P65 protein in cultured astrocytes by Western blot, and then further probed the translocation of NF-κB/P65 using immunofluorescence and the contents of TNF-α, IL-1β and Aβ in culture supernatant through ELISA. We found that all of these indexes increased at different degrees after LPS-stimulation. However, if pretreatment with anti- TLR4 antibody, such stimulating effects of LPS on the nuclear translocation of NF-κB/P65 and TNF-α, IL-1β, Aβ contents in astrocytic culture supernatant were reduced significantly or disappeared in comparison with the group with only LPS-administration. Our results suggest that TLR4 in astrocytes might play an important role in the inflammation and Aβ formation through the TLR4/NF-κB signaling pathway, thus providing new knowledge and understanding of the inflammatory hypothesis of AD pathogenesis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Signal Transduction
/
Cells, Cultured
/
Cerebral Cortex
/
Astrocytes
/
Aspartic Acid Endopeptidases
/
Tumor Necrosis Factor-alpha
/
Amyloid beta-Protein Precursor
/
Cell Biology
/
Transcription Factor RelA
Limits:
Animals
Language:
English
Journal:
Acta Physiologica Sinica
Year:
2014
Type:
Article
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