The progressive study on gene therapy for hyperphenylalaninemia rats / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 713-717, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256133
ABSTRACT
To construct a new high effective genetic engineering strain which can express active PAL enzyme in Lactococcus lactis (L.L), and acquire better effect on curing hyperphenylalaninemia rats, Firstly translational fusion vector and transcriptional fusion vector were constructed in E. coli MC1061, and then PAL cDNA was transformed into L.L. Two kinds of high effect strain were compared with their enzyme activity and animal experiment was carried out. The results showed (1) Two kinds of engineering L.L. were obtained and translational fusion strain has higher level enzyme activity. (2) The amount of transcinnamic aicd reach peak when induced for 6 hours. (3) The blood phe level of the treated rats was significantly reduced compared with non-treated rats when receiving fresh p(NZ8048-PAL)1/NZ9000. The engineering L.L(translational fusion strain) can significantly reduce the blood phe level of the hyperphenylalaninemia rats, which has more superiority than pMG36e-PAL/L. L.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenylalanine Ammonia-Lyase
/
Phenylketonurias
/
Therapeutics
/
Genetic Therapy
/
Polymerase Chain Reaction
/
Lactococcus lactis
/
Rats, Wistar
/
Escherichia coli
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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