Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 703-708, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256135
ABSTRACT
Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apoproteins
/
Pharmacology
/
Substrate Specificity
/
Bacterial Proteins
/
Proteins
/
Catalysis
/
Cyanobacteria
/
Octoxynol
/
Light-Harvesting Protein Complexes
/
Lyases
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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