The PTD domain of Tat protein enhance GFP protein delivering into myeloma cell SP2/0 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 644-647, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256145
ABSTRACT
In order to detect the protein delivery mediated by the PTD (protein transduction domain) of TAT Protein, a expression vector, named pT7460-GFP, was constructed by insert the PTD DNA Sequence, followed by a GFP (green fluorescent protein) gene fused in-frame, into the pT7450 vector. The TAT-GFP fusion protein was expressed in the E. coli ER2566. Most of the fusion protein was presented in the inclusion body. The protein was purified by Ni2+ affinity chromatography under denature conditions, then by a Sepharose Q column to remove urea. The soluble denatured protein was added directly to medium containing the Myeloma Cell SP2/0. It came out that the fusion protein could be detected delivered into the cells under fluorescent microscope in a short time.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Tumor Cells, Cultured
/
Gene Products, tat
/
Chemistry
/
Protein Transport
/
Green Fluorescent Proteins
/
Luminescent Proteins
/
Metabolism
/
Microscopy, Fluorescence
/
Multiple Myeloma
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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