Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 626-629, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256150
ABSTRACT
The effect of the calcium ion (Ca2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2 mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8%, 30.8%, 5.1%, and 26.8%, respectively. However, the Ca2+ concentrations in the medium above 0.6 mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Keratinocytes
/
Cell Adhesion
/
Cell Differentiation
/
Cell Division
/
Cells, Cultured
/
Calcium
/
Cellular Senescence
/
Cell Biology
/
Epidermis
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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