Cloning of the major antigen region of E2 gene of hog cholera virus and expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 605-608, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256155
ABSTRACT
The major antigen region of E2 gene of Hog Cholera Prevalent Strain (Guangxi Yuling Strain) and Chinese Hog Cholera Lapinised Virus (C-strain) derived from hog and rabbit spleen tissue, was amplified by reverse transcription polymerase chain reaction(RT-PCR) and the nested Polymerase Chain Reaction (nPCR). After the amplified fragments were cloned into the expression vector pPROEX-HTb, the recombinant plasmids pPROEX-GXYL and pPROEX-C were obtained. The insert position, the size and the reading frame were right by PCR, restriction digestion and the sequence analysis. SDS-PAGE indicated that both of the reciepient germs transducted and induced by the recombinant plasmids pPROEX-GXYL and pPROEX-C could express the major antigen region of E2 gene. Western-blot indicated that the expressed antigen protein could be recognized by the positive serum of CSFV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Viral Envelope Proteins
/
Blotting, Western
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
Allergy and Immunology
/
Escherichia coli
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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