Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 551-555, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256166
ABSTRACT
Hepatitis B virus MHBst and HBx fragments were amplified to construct eukaryotic expression vector pCDNA3.1-MHBst and pCDNA3.1-HBx. ST3GalI promoter region was obtained by the method of PCR and GFP report plasmid pEGFP-N1-Psial was constructed. pCDNA3.1-MHBst or pCDNA3.1-HBx with pEGFP-N1-Psial were transiently co-transfected into QGY-7701 cells using calcium phosphate-DNA co-precipitation, respectively. The expressions of Psial-directed GFP were analyzed by FAC-Scalibur. It was found that MHBst/HBx could upregulate ST3GalI promoter activity by 35.2% and 43.8%, respectively. We report the regulation of ST3GalI by MHBst and HBx transactivators. It would be helpful to further investigate the relation between hepatitis B virus infection and sialyltransferase expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Sialyltransferases
/
Transfection
/
Gene Expression Regulation, Enzymologic
/
Trans-Activators
/
Hepatitis B virus
/
Promoter Regions, Genetic
/
Genetics
/
Hepatitis B Surface Antigens
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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