Expression of cre gene in Escherichia coli and bioassay its expression product / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 497-500, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256177
ABSTRACT
The Cre recombinase from bacteriophage P1 can recognize specific DNA sequences, cleave DNA at specific target sites, and then ligate it to the cleaved DNA of a second site. In this study, cre gene was cloned into the pGEM-T Easy vector via PCR procedure. Then the cre gene was inserted into an expression vector pET-29a and expressed in E. coli BL21 (DE3). A 38 kD soluble protein was expressed and named CRE. CRE was purified by DEAE-52 chromatography. Bioassay of the partially purified product showed that CRE can cleave the plasmid pGLGFP which contains two loxP site with the same direction.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Viral Proteins
/
Recombinant Proteins
/
Gene Expression Regulation, Enzymologic
/
Chromatography, DEAE-Cellulose
/
Integrases
/
Green Fluorescent Proteins
/
Escherichia coli
/
Genetics
/
Luminescent Proteins
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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