The changes of rare codons increase expression level of SEA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 477-480, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256181
ABSTRACT
The rare codons of a fragment in staphylococcal enterotoxin A gene were turned into the most high usage frequency codons in E. coli by overlap PCR technique. Genes of sea and seam were cloned into 7ZTS expression vector and transformed into JM109(DE3), respectively. The result shows that expression level of sea gene was very low, but the expression level of seam was as high as 15% of total cell proteins. The expression product shows activity of antitumor in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Recombinant Proteins
/
Codon
/
Molecular Sequence Data
/
Base Sequence
/
Gene Expression Regulation, Bacterial
/
Point Mutation
/
Drug Therapy
/
Electrophoresis, Polyacrylamide Gel
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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