Expression of polyprotein of infectious bursal disease virus in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 472-476, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256182
ABSTRACT
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bombyx
/
Viral Proteins
/
Virology
/
Enzyme-Linked Immunosorbent Assay
/
Gene Expression
/
Blotting, Western
/
Infectious bursal disease virus
/
Polyproteins
/
Electrophoresis, Polyacrylamide Gel
/
Genetic Vectors
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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