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Expression of polyprotein of infectious bursal disease virus in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology ; (12): 472-476, 2002.
Article in Chinese | WPRIM | ID: wpr-256182
ABSTRACT
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Bombyx / Viral Proteins / Virology / Enzyme-Linked Immunosorbent Assay / Gene Expression / Blotting, Western / Infectious bursal disease virus / Polyproteins / Electrophoresis, Polyacrylamide Gel / Genetic Vectors Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2002 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bombyx / Viral Proteins / Virology / Enzyme-Linked Immunosorbent Assay / Gene Expression / Blotting, Western / Infectious bursal disease virus / Polyproteins / Electrophoresis, Polyacrylamide Gel / Genetic Vectors Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2002 Type: Article