The construction of shuttle vectors of Brevibacillus brevis-Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 438-441, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-256189
ABSTRACT
The 5' region of the cell wall protein(CWP) gene containing multiple tandem promoters and the signal peptide-coding sequence was isolated by PCR from Br. brevis 50, and used to construct the shuttle vector pBKE50, which included the replication origin of pUB110 and the erythromycin-resistance gene of pGK12. The alpha-amylase gene of Bacillus subtilis 168 was ligated to pBKE50, producing plasmid pBKE50/alpha-amy. After the resulting plasmid was introduced into Br. brevis 50, soluble and biologically active alpha-amylase was secreted directly into the culture medium. The expression level of alpha-amylase in the recombinant Br. brevis 50 was twice higher than that of the donor strain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plasmids
/
Bacillus
/
Recombinant Fusion Proteins
/
Drug Resistance, Microbial
/
Erythromycin
/
Cloning, Molecular
/
Escherichia coli
/
Alpha-Amylases
/
Genetic Vectors
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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