Immunohistochemistry using epidermal growth factor receptor mutation-specific antibodies of delE746-A750 and L858R in lung adenocarcinomas / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 173-177, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-256226
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the immunohistochemical detection of epidermal growth factor receptor(EGFR) mutations using two EGFR mutation-specific monoclonal antibodies delE746-A750 and L858R.</p><p><b>METHODS</b>A total of 175 paraffin-embedded lung adenocarcinoma tissue samples previously genotyped by directive DNA sequencing were subject to immunostaining using delE746-A750 and L858R antibodies.</p><p><b>RESULTS</b>There was no significant difference of mutation detection between DNA sequence analysis and delE746-A750 and/or L858R immunostaining (33.7% vs 30.9%, P > 0.05). The overall sensitivity, specificity, positive predictive value and negative predictive value of immunostaining using these two EGFR mutation-specific antibodies were 83.1%, 95.7%, 90.7% and 90.9%, respectively.</p><p><b>CONCLUSION</b>With high sensitivity and good specificity, immunohistochemistry using EGFR mutation-specific monoclonal antibodies is an adequate, easy and cost-effective prescreening method to detect EGFR mutations using paraffin-embedded tissue specimens of lung adenocarcinomas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
DNA Mutational Analysis
/
Immunohistochemistry
/
Adenocarcinoma
/
Sensitivity and Specificity
/
Paraffin Embedding
/
Point Mutation
/
Gene Deletion
/
Allergy and Immunology
/
ErbB Receptors
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2013
Type:
Article
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