The fibroblast primary cell culture by the split-thickness skin slide technique / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To acquire lots of cell to culture during the primary cell culture.</p><p><b>METHOD</b>We take the split-thickness skin slide technique to acquire the dissociated fibroblast cell in two big-ear rats.</p><p><b>RESULTS</b>The cell number is above 10(6) from 1 cm x 2 cm split-thickness skin slide and the technique is simple, economic, effectve.</p><p><b>CONCLUSION</b>We think this way is better than other methods, and should be adopted in the primary cell culture, especially in fibroblast transplantation by injection.</p>