Effects of serum anti-sperm antibodies positive on the Fas/Fas-L apoptosis pathway in the testis tissue and testicular germ cells of pubertal male rats / 中华男科学杂志
National Journal of Andrology
;
(12): 111-115, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-256953
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rat model of anti-sperm antibody (AsAb)-mediated immune infertility, and investigate the effects of serum AsAb positive on the Fas/Fas-L apoptosis pathway in testis tissue and testicular germ cells of pubertal male rats.</p><p><b>METHODS</b>Thirty 5-week-old Wistar male rats were included in this study, 10 killed for preparation of sperm suspension, 10 as normal controls, and the other 10 made models of AsAb-positive immune infertility (experimental group). Four weeks after modeling, the testes of the rats were harvested for observation of the changes in the testis tissue under the light microscope and detection of the expressions of Fas, Fas-L and Caspase-3 proteins by immunohistochemistry.</p><p><b>RESULTS</b>Compared with the control group, the experimental group showed obvious apoptotic changes in the testis tissue and remarkably increased expressions (OD value) of Fas (161.87 +/- 5.37 vs 176.97 +/- 4.58), Fas-L (150.27 +/- 8.65 vs 187.52 +/- 7.76) and Caspase-3 (120.37 +/- 6.76 vs 157.65 +/- 7.38) (P < 0.01).</p><p><b>CONCLUSION</b>Serum AsAb affected the infertility of pubertal male rats, and its mechanisms might be associated with up-regulated expression of Fas, Fas-L and Caspase-3 proteins in the Fas/Fas-L apoptotic pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autoantibodies
/
Testis
/
Signal Transduction
/
Rats, Wistar
/
Apoptosis
/
Fas Receptor
/
Cell Biology
/
Allergy and Immunology
/
Caspase 3
/
Fas Ligand Protein
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2013
Type:
Article
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