Application of TRUST, TP-ELISA and TPPA in the serologic diagnosis of syphilis / 中华男科学杂志
National Journal of Andrology
;
(12): 1020-1022, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-256998
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the application value of toluidine red unheated serum test (TRUST), treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum particle agglutination assay (TPPA) in the serologic diagnosis of syphilis.</p><p><b>METHODS</b>We collected serum samples from 12 622 inpatients, 2 253 outpatients and 1 500 subjects in the medical examination center, and screened them by TRUST and TP-ELISA, followed by quantitative tests using TRUST and TPPA.</p><p><b>RESULTS</b>Among the 16 375 cases, clinically diagnosed syphilis was confirmed in 384, with a positive rate of 100% either by TRUST (12 -116) or TPPA (180 - 12 560), and 92.2% by TP-ELISA.</p><p><b>CONCLUSION</b>TP-ELISA and TRUST should be simultaneously used for syphilis screening, but not TP-ELISA alone, and the results should be confirmed by TPPA. The combination of the three methods plays a valuable role in reducing the misdiagnosis and missed diagnosis, and contributes to the early and accurate diagnosis of syphilis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Treponema pallidum
/
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Syphilis Serodiagnosis
/
Syphilis
/
Diagnosis
/
Methods
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Child, preschool
/
Humans
/
Infant
/
Infant, Newborn
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2012
Type:
Article
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