Effects of SUMO specific protease 1 on hPXR-mediated P-gp gene expression / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1192-1196, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-257007
ABSTRACT
The study aimed to investigate the effects of small ubiquitin-related modifier (SUMO) specific protease 1 (SENP1) on human PXR-mediated MDR1 transcriptional activity and mRNA expression. Empty vector and expression plasmids, including PXR, SENP1 and SENP1 mutant (SENP1m) were transiently transfected into HepG2 and LS174T cells using Lipo2000. Transcriptional activity was detected by dual luciferase reporter gene assay, and mRNA level was measured using real-time polymerase chain reaction. The results showed that SENP1 could remarkably reduce the rifampicin (RIF)-induced MDR1 reporter activity and mRNA level in hPXR over expressed HepG2 and LS174T cells (P < 0.05), whereas adding SENP1m restored the RIF-induced increases (P < 0.05). These results indicated that SENP1 could repress the RIF-induced hPXR-mediated MDR1 transcriptional activity and mRNA expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endopeptidases
/
RNA, Messenger
/
Cysteine Endopeptidases
/
Gene Expression
/
Transcriptional Activation
/
Receptors, Cytoplasmic and Nuclear
/
ATP Binding Cassette Transporter, Subfamily B
/
Hep G2 Cells
/
Peroxisome-Targeting Signal 1 Receptor
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article
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