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The expression of BmK AngM1 in Mut(s) and Mut(+) recombinants of Pichia pastoris / 药学学报
Acta Pharmaceutica Sinica ; (12): 910-915, 2015.
Article in Chinese | WPRIM | ID: wpr-257047
ABSTRACT
BmK AngM1 is a long-chain scorpion toxin purified from the venom of Buthus martensii Karsch. It has been reported to exhibit evident analgesic effect and low toxicity, and has the potential to be a novel analgesic drug. The BmKAngM1 gene was transformed into Pichiapastoris GS115. Mut+ and Mut(s) recombinant strains were screened by phenotype and Mut+ recombinant strains were used to detect BmK AngMl gene copy number in the real-time PCR. Expression of BmK AngM1 in the Mut+ recombinant strain was compared with that of the Mut(s) recombinant strain with the same single copy of BmK AngM1 gene under the same condition. The results indicated that the transcription level of BmK AngM1 gene in the Mut(s) recombinant strain was 2.7 fold of that in the Mut recombinant strain in the real-time PCR, and the expression of BmK AngM 1 in the Mut(s) recombinant strain was 1.5 fold of that in the Mut+ recombinant strain. Therefore, Mut(s) recombinant strain showed better ability to express BmK AngM1 than Mut+ recombinant strain.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pichia / Scorpion Venoms / Recombinant Proteins / Chemistry / Gene Dosage / Arthropod Proteins / Metabolism Limits: Animals Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pichia / Scorpion Venoms / Recombinant Proteins / Chemistry / Gene Dosage / Arthropod Proteins / Metabolism Limits: Animals Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2015 Type: Article