Identification of medicinal plant Dendrobium based on the chloroplast psbK-psbI intergenic spacer / 药学学报
Acta Pharmaceutica Sinica
; (12): 783-787, 2015.
Article
in Zh
| WPRIM
| ID: wpr-257067
Responsible library:
WPRO
ABSTRACT
In this paper, the chloroplast psbK-psbI intergenic spacers of 18 species of Dendrobium and their adulterants were amplified and sequenced, and then the sequence characteristics were analyzed. The sequence lengths of chloroplast psbK-psbI regions of Dendrobium ranged from 474 to 513 bp and the GC contents were 25.4%-27.6%. The variable sites were 71 while the informative sites were 46. The inter-specific genetic distances calculated by Kimura 2-parameter (K2P) of Dendrobium were 0.006 1-0.058 1, with an average of 0.028 4. The K2P genetic distances between Dendrobium species and Bulbophyllum odoratissimum were 0.093 2-0.120 4. The NJ tree showed that the Dendrobium species can be easily differentiated from each other and 6 samples of the inspected Dendrobium species were identified successfully through sequencing the psbK-psbI intergenic spacer. Therefore, the chloroplast psbK-psbI intergenic spacer can be used as a candidate marker to identify Dendrobium species and its adulterants.
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
/
Chloroplasts
/
Classification
/
DNA, Plant
/
DNA, Chloroplast
/
DNA, Ribosomal Spacer
/
Dendrobium
/
Genetics
Type of study:
Prognostic_studies
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article