Rapid N-glycosylation profiling of glycoprotein based on ultrafiltration-devices / 药学学报
Acta Pharmaceutica Sinica
;
(12): 755-759, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-257071
ABSTRACT
With development of bio-technique, more and more proteins were applied as clinical approaches. However, the protein homogeneity, especially the N-glycosylation limited the further research and application of these protein drugs. The analysis method for N-glycans is believed to be critical in protein drugs development. To enhance the N-glycans isolation efficiency and accelerate the pretreatment, a new strategy was built on ultrafiltration-devices. New methods increased the isolation efficiency of N-glycans containing N-acetylglucosa mine with 10%-20%. The degrading of N-glycans containing sialic acids was also minimized with this method. 20%-100% more N-glycans with sialic acids were isolated. The pretreatment was finished within 30 min. Coupled with HPLC-HRMS, an effective and reliable strategy designed for protein drugs N-glycans analysis were developed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polysaccharides
/
Glycosylation
/
Glycoproteins
/
Ultrafiltration
/
Chemistry
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article
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