Analysis and identification of water soluble components of water buffalo horn / 药学学报
Acta Pharmaceutica Sinica
;
(12): 594-598, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-257095
ABSTRACT
This study is to analyze and identify the water soluble components of water buffalo horn (Bubali Cornu, WBH), and also establish a method for investigating these components. Shotgun proteomic analysis identified proteins in WBH aqueous extraction keratin, collagen, desmoglein, etc. Ultrafiltration and LC-MS/MS were used to separate and identify the peptides in WBH aqueous extract, as a result, identified peptides were mainly derived from nonspecific degradation products of keratin and collagen, which including C-terminal peptides and non C-terminal peptides. Hypoxanthine, uridine, guanosine, and adenosine were identified by comparing with the standards. The strategy in present study could be used in analyzing water soluble components of animal horn derived TCM. It provides a reference for investigation of the material basis of animal horn derived TCM.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Mass Spectrometry
/
Uridine
/
Buffaloes
/
Chemistry
/
Chromatography, Liquid
/
Hypoxanthine
/
Proteomics
/
Tandem Mass Spectrometry
/
Guanosine
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article
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