Study on the expression of DNA excision repair biomarkers in cispatin-treated lung cancer cell line / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 109-111, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-257221
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression levels of ERCC2, UDG, and PCNA in cisplatin-treated A549 cell line.</p><p><b>METHOD</b>Comet assay, RT-PCR, and western blot were used to study the mRNA and protein expression levels of ERCC2, UDG, and PCNA.</p><p><b>RESULTS</b>When treated with IC(20) cisplatin, the DNA damage level increased as the cisplatin treated time increased within 24 h of cisplatin treatment. The tail state 12 h and 24 h after treatment was 5.02 +/- 0.68 and 7.22 +/- 0.53 respectively, which was significantly higher than those of the controls (2.73 +/- 0.29). The tail state 24 h after treatment was not significantly different from that of the controls. The DNA damage level decreased to normal after cisplatin treatment in 24 h (tail state 3.64 +/- 0.7). The expression levels of ERCC2, UDG, PCNA protein (4.37 +/- 0.57, 5.47 +/- 0.46, 2.21 +/- 0.47 respectively) and mRNA (0.71 +/- 0.08, 0.74 +/- 0.06, 0.82 +/- 0.09) were increased significantly within 24 h exposure and decreased to normal 24 h after cisplatin treatment. The 3 enzymes' mRNA and protein expression increased when treated with cisplatin, but the changes of protein level were slower than those of mRNA levels.</p><p><b>CONCLUSIONS</b>The DNA repair capability in A549 cells increases after cisplatin treatment. Cisplatin was a positive regulation of ERCC2, UDG, PCNA expression levels, which causes the increase of mRNA, and protein. The positive regulation only works in a short time and returns normal after 24 h of cisplatin treatment.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Transcription Factors
/
Protein Biosynthesis
/
RNA, Messenger
/
Proteins
/
Biomarkers, Tumor
/
Gene Expression Regulation, Neoplastic
/
Cisplatin
/
DNA Helicases
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS