Effects of selenium and fluoride on apoptosis and lipid peroxidation in human hepatocytes / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 235-238, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-257258
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of selenium and fluoride on apoptosis and lipid peroxidation in human hepatocytes in vitro.</p><p><b>METHODS</b>The apoptosis, cell cycle, GSH content and lipid peroxides (LPO) level in human hepatocytes, LPO level and LDH, AST and ALT activity in cell culture supernatants were investigated after hepatocytes were incubated with selenium and/or fluoride for around 12 hours periods in vitro.</p><p><b>RESULTS</b>The percentage of hepatocyte apoptosis bodies (15.557 +/- 2.056)%, the number of cells in S phase (4.823 +/- 0.454)% and LPO level in liver tissue and supernatant [(2.884 +/- 0.589) and (3.547 +/- 0.561) nmol/L MDA/mg.prot, respectively], AST and LDH activity in supernatants (91.1 +/- 36.4 and 140.4 +/- 7.6 U/L, respectively) in the fluoride treated group was higher than the control group [(10.313 +/- 1.023)%, (3.253 +/- 0.743)%, (1.473 +/- 0.401) nmol/L MDA/mg.prot, (1.694 +/- 0.443) nmol/L MDA/mg.prot, (54.5 +/- 3.2) U/L and (126.4 +/- 2.6) U/L, respectively], The GSH content in live tissue [(4.225 +/- 0.781) microgram/mg.prot] is lower than control group [(7.595 +/- 1.042) microgram/mg.prot]. Selenium treatment reduced these kinds of toxicity of fluoride through raising GSH content, reducing LPO level, LDH and AST activity and percentage of apoptosis bodies.</p><p><b>CONCLUSIONS</b>Selenium can antagonist apoptosis and lipid peroxidation of hepatocytes induced by fluoride.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Aspartate Aminotransferases
/
Selenium
/
Time Factors
/
Lipid Peroxidation
/
Cell Cycle
/
Cells, Cultured
/
Apoptosis
/
Hepatocytes
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS