Studies on DNA damage and apoptosis in rat brain induced by fluoride / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 222-224, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-257262
ABSTRACT
<p><b>OBJECTIVE</b>To explore the DNA damage effects and apoptosis in brain cells of rats induced by sodium fluoride.</p><p><b>METHODS</b>SD rats were divided into two groups, i.e. control group and fluoride treated group, which were injected intraperitoneally with distilled water and sodium fluoride (20 mg.kg(-1).d(-1)) respectively. On the hand, 5 mmol/L NaF were used in in vitro study. Single Cell Gel Electrophosis (SCGE or Comet Assay) was utilized to measured DNA damage and apoptosis was detected by the TUNEL method and Flow Cytometry (FCM).</p><p><b>RESULTS</b>The DNA damage in pallium neurons in rats of the fluoride group was much more serious compared with those of the control group, with the Ridit value being 0.351 and 0.639 respectively (P < 0.01) in vivo, and 0.384 4 and 0.650 1 respectively (P < 0.01) in vitro. TUNEL positive cells were found in pallium, hippocampus and cerebellar granule cells in rats of fluoride group, whereas those in the control group were rare. It was demonstrated by FCM results that the percentages of apoptotic cells both in pallium and hippocampus were significantly higher (P < 0.01) in rats of fluoride group (27.12 +/- 3.08, 34.97 +/- 5.46) than those in control group (4.63 +/- 0.98, 5.35 +/- 0.79), (P < 0.01).</p><p><b>CONCLUSION</b>Sodium fluoride could induce DNA damage and apoptosis in rats brain.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Sodium Fluoride
/
Brain
/
DNA
/
Rats, Sprague-Dawley
/
Apoptosis
/
In Situ Nick-End Labeling
/
Comet Assay
/
Flow Cytometry
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2002
Type:
Article
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