Effects of (-)-epigallocatechin-3-gallate on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibroblasts irradiated with ultraviolet A / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1838-1841, 2004.
Article
in English
| WPRIM
| ID: wpr-257349
ABSTRACT
<p><b>BACKGROUND</b>It is known that ultraviolet irradiation can affect cellular function through a number of signaling pathways. (-)-epigallocatechin-3-gallate (EGCG) is the major effective component in green tea and can offer protection from ultraviolet-induced damage. In this study, we investigated the protective mechanism of EGCG on human dermal fibroblasts damaged by ultraviolet A (UVA) in vitro.</p><p><b>METHODS</b>Transcription factor Jun protein levels were measured by Western blot. Matrix metalloproteinase 1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA were studied by reverse transcription-polymerase chain reaction (RT-PCR) analysis in conjunction with computer-assisted image analysis. MMP-1 and TIMP-1 proteins were quantified by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>EGCG decreased transcription activity of Jun protein after induction by UVA. Both the mRNA and protein levels of MMP-1 were increased by UVA irradiation, while no significant changes were observed in TIMP-1 levels. The ratio of MMP-1 to TIMP-1 showed statistically significant differences compared with the control. EGCG decreased the ratio of MMP-1 to TIMP-1 by inhibiting UVA-induced MMP-1 expression (P < 0.05).</p><p><b>CONCLUSION</b>EGCG can protect human fibroblasts against UVA damage by downregulating the transcription activity of Jun protein and the expression of MMP-1. The ratio of MMP-1 to TIMP-1, rather than the levels of MMP-1 or TIMP-1 alone, may play a significant role in human skin photodamage.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Radiation Effects
/
Radiation-Protective Agents
/
Ultraviolet Rays
/
RNA, Messenger
/
Catechin
/
Cells, Cultured
/
Gene Expression Regulation
/
Proto-Oncogene Proteins c-jun
/
Tissue Inhibitor of Metalloproteinase-1
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2004
Type:
Article
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